排序方式: 共有167条查询结果,搜索用时 15 毫秒
61.
62.
Josefa Gómez‐Maldonado Isabelle Lesur Noe Fernández‐Pozo Marina Rueda‐López Dario Guerrero‐Fernández Vanessa Castro‐Rodríguez Hicham Benzekri Rafael A. Cañas María‐Angeles Guevara Andreia Rodrigues Pedro Seoane Caroline Teyssier Alexandre Morel François Ehrenmann Grégoire Le Provost Céline Lalanne Céline Noirot Christophe Klopp Isabelle Reymond Angel García‐Gutiérrez Jean‐François Trontin Marie‐Anne Lelu‐Walter Celia Miguel María Teresa Cervera Francisco R. Cantón Christophe Plomion Luc Harvengt Concepción Avila M. Gonzalo Claros Francisco M. Cánovas 《Plant biotechnology journal》2014,12(3):286-299
63.
Li TF Chen D Wu Q Chen M Sheu TJ Schwarz EM Drissi H Zuscik M O'Keefe RJ 《The Journal of biological chemistry》2006,281(30):21296-21304
Transforming growth factor-beta (TGF-beta) plays an essential role in chondrocyte maturation. It stimulates chondrocyte proliferation but inhibits chondrocyte differentiation. In this study, we found that TGF-beta rapidly induced beta-catenin protein levels and signaling in murine neonatal sternal primary chondrocytes. TGF-beta-increased beta-catenin induction was reproduced by overexpression of SMAD3 and was absent in Smad3(-/-) chondrocytes treated with TGF-beta. SMAD3 inhibited beta-transducin repeat-containing protein-mediated degradation of beta-catenin and immunoprecipitated with beta-catenin following TGF-beta treatment. Both SMAD3 and beta-catenin co-localized to the nucleus after TGF-beta treatment. Although both TGF-beta and beta-catenin stimulated cyclin D(1) expression in chondrocytes, the effect of TGF-beta was inhibited with beta-catenin gene deletion or SMAD3 loss of function. These results demonstrate that TGF-beta stimulates cyclin D(1) expression at least in part through activation of beta-catenin signaling. 相似文献
64.
Cathy Poillot Kaouthar Dridi Hicham Bichraoui Julien Pêcher Sebastien Alphonse Badreddine Douzi Michel Ronjat Hervé Darbon Michel De Waard 《The Journal of biological chemistry》2010,285(44):34168-34180
Maurocalcine has been the first demonstrated animal toxin acting as a cell-penetrating peptide. Although it possesses competitive advantages, its use as a cell-penetrating peptide (CPP) requires that analogues be developed that lack its characteristic pharmacological activity on ryanodine-sensitive calcium channels without affecting its cell-penetrating and vector efficiencies. Here, we present the synthesis, three-dimensional 1H NMR structure, and activity of d-maurocalcine. We demonstrate that it possesses all of the desired features for an excellent CPP: preserved structure, lack of pharmacological action, conserved vector properties, and absence of cell toxicity. This is the first report of a folded/oxidized animal toxin in its d-diastereomer conformation for use as a CPP. The protease resistance of this new peptide analogue, combined with its efficient cell penetration at concentrations devoid of cell toxicity, suggests that d-maurocalcine should be an excellent vector for in vivo applications. 相似文献
65.
Jos H.M. Lange Martina A.W. van der Neut Henri C. Wals Gijs D. Kuil Alice J.M. Borst Arie Mulder Arnold P. den Hartog Hicham Zilaout Wouter Goutier Herman H. van Stuivenberg Bernard J. van Vliet 《Bioorganic & medicinal chemistry letters》2010,20(3):1084-1089
The synthesis and structure–activity relationship studies of imidazoles are described. The target compounds 6–20 represent a novel chemotype of potent and CB2/CB1 selective cannabinoid CB2 receptor antagonists/inverse agonists with very high binding efficiencies in combination with favourable log P and calculated polar surface area values. Compound 12 exhibited the highest CB2 receptor affinity (Ki = 1.03 nM) in this series, as well as the highest CB2/CB1 subtype selectivity (>9708-fold). 相似文献
66.
Jos H.M. Lange Amos Attali Martina A.W. van der Neut Henri C. Wals Arie Mulder Hicham Zilaout Ate Duursma Hans H.M. van Aken Bernard J. van Vliet 《Bioorganic & medicinal chemistry letters》2010,20(17):4992-4998
The synthesis and SAR of 3-alkyl-4-aryl-4,5-dihydropyrazole-1-carboxamides 1–23 and 1-alkyl-5-aryl-4,5-dihydropyrazole-3-carboxamides 24–27 as two novel cannabinoid CB1 receptor agonist classes were described. The target compounds elicited high affinities to the CB1 as well as the CB2 receptor and were found to act as CB1 receptor agonists. The key compound 19 elicited potent CB1 agonistic and CB2 inverse agonistic properties in vitro and showed in vivo activity in a rodent model for multiple sclerosis after oral administration. 相似文献
67.
68.
Hicham El Miri Mohamed Rhajaoui Oumnia Himmi Souâd Ouahabi Abdelaziz Benhoussa Chafika Faraj 《法国昆虫学会纪事》2013,49(2):154-159
Résumé. La leishmaniose cutanée est un important problème de santé publique dans la province de Sidi Kacem au nord-ouest du Maroc. L'incidence de cette affetion, due aussi bien à Leishmania tropica qu'à L. infantum est en augmentation progressive depuis 1997. La présente étude a été menée dans le but d'étudier l'écologie et la dynamique des populations des vecteurs de cette maladie dans les principaux foyers de cette province. Un total de 4504 spécimens appartenant à deux genres et six espèces a été capturé à l'aide de pièges adhésifs et lumineux, dans cinq foyers. Les espèces dominantes sont Phlebotomus sergenti représentant 42,6% du total des phlébotomes capturés et P. longicuspis avec 27,2% des captures. Ces deux espèces sont présentes de mai à novembre et évoluent en deux générations avec deux pics de densité, le premier en juin et le second en septembre. En considérant la grande spécificité vecteur-parasite connue en épidémiologie des leishmanioses ainsi que l'abondance des espèces capturées pendant la saison favorable à la transmission, P. sergenti et P. longicuspis seraient respectivement les vecteurs les plus probables des leishmanioses à L. tropica et à L. infantum dans la région étudiée. Le risque de transmission de la maladie serait plus grand durant le deuxième pic de densité en fin été- début automne. 相似文献
69.
Khalid El Allali Hicham Farsi Mohammed Piro Mohamed Rachid Achaâban Mohammed Ouassat Etienne Challet 《Chronobiology international》2013,30(8):1047-1057
ABSTRACTDaily pattern of locomotor activity (LA), one of the most studied rhythms in humans and rodents, has not been widely investigated in large mammals. This is partly due to the high cost and breakability of used automatic devices. Since last decade, smartphones are becoming ubiquitous. Meanwhile, several applications detecting activity by using internal sensors were made available. In this study, we assumed that this device could be a cheaper and easier way to measure the LA rhythm in humans and large mammals, like camel and goat. A smartphone application (Nokia Mate Health), normally used to quantify physical activities in humans, was chosen for the study. To validate the rhythm data obtained from the smartphone, LA rhythm was simultaneously recorded using an automatic device, the Actiwatch-Mini®. Results showed that the smartphone provided a clear and significant daily rhythm of LA. The visual assessment of the superimposed LA rhythm’s curves in all three species showed that the smartphone application displayed similar rhythms as those recorded by the Actiwatch-Mini. Highly significant positive correlation (p≤ 0.0001) exists between the two recording rhythms. The daily periods were both the same at 24.0 h. Acrophases were also significantly similar and occurring around mid-day: 11:40 ± 0.35 h vs 11:41 ± 0.35 h for the camel, 11:25 ± 0.19 h vs 11:37 ± 0.25 h for the goat and 13:04 ± 0.11 h vs 13:51 ± 0.28 h for humans using smartphone and Actiwatch, respectively. The related mesor and amplitude were also close between the two recording devices. Results indicate clearly that using smartphones constitutes a reliable cheap tool to study LA rhythm for chronobiology studies, especially in laboratories facing lack of funding. 相似文献
70.
Haouane H El Bakkali A Moukhli A Tollon C Santoni S Oukabli A El Modafar C Khadari B 《Genetica》2011,139(9):1083-1094
The conservation of cultivated plants in ex-situ collections is essential for the optimal management and use of their genetic
resources. For the olive tree, two world germplasm banks (OWGB) are presently established, in Córdoba (Spain) and Marrakech
(Morocco). This latter was recently founded and includes 561 accessions from 14 Mediterranean countries. Using 12 nuclear
microsatellites (SSRs) and three chloroplast DNA markers, this collection was characterised to examine the structure of the
genetic diversity and propose a set of olive accessions encompassing the whole Mediterranean allelic diversity range. We identified
505 SSR profiles based on a total of 210 alleles. Based on these markers, the genetic diversity was similar to that of cultivars
and wild olives which were previously characterised in another study indicating that OWGB Marrakech is representative of Mediterranean
olive germplasm. Using a model-based Bayesian clustering method and principal components analysis, this OWGB was structured
into three main gene pools corresponding to eastern, central and western parts of the Mediterranean Basin. We proposed 10
cores of 67 accessions capturing all detected alleles and 10 cores of 58 accessions capturing the 186 alleles observed more
than once. In each of the 10 cores, a set of 40 accessions was identical, whereas the remaining accessions were different,
indicating the need to include complementary criteria such as phenotypic adaptive and agronomic traits. Our study generated
a molecular database for the entire OWGB Marrakech that may be used to optimise a strategy for the management of olive genetic
resources and their use for subsequent genetic and genomic olive breeding. 相似文献